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  • Molecular Biology Reagents

    Expand your control and reduce variables, making your workflow as efficient and reliable as possible, with J.T. Baker® ULTRAPURE Bioreagents.

    ULTRAPURE bioreagents biological buffers are non-toxic to cells and are not absorbed through cell membranes. They are zwitterionic, contributing negligible ionic strength while providing high buffering capacity.

    Bioreagents  

    • Assay purity ranging from 98% to above 99.9%
    • No detectable DNase, RNase and protease
    • Low heavy metal content
    • Low insolubles

    ULTRAPURE Bioreagents Biological Buffers   

    Product   Product Number Product  Product Number
    Acrylamide  4081 2-Mercaptoethanol  4049
    Acrylamide/Bis, 37.5:1, 40% Solution  4970 MES, Monohydrate, Free Acid, Crystalline  4014
    Acrylamide/Bis, 29:1, 40% Solution  4969 N,N'-Methylenebisacrylamide  4031
    Acrylamide/Bis, 19:1, 40% Solution  4968 MOPS  4004
    8% Premix Acrylamide Solution  4170 MOPS, Sodium Salt  4163
    6% Premix Acrylamide Solution  4169 Phenol, White Fused Crystal  4056
    Agarose, Low Melting  4090 PIPES  4265
    Agarose, PFGE  4063 PIPES, Sodium Salt  4266
    Agarose, Standard, Low Electroendosmosis (EEO)  A426 Potassium Acetate, Crystal  2915
    Ammonium Persulfate  4030 Potassium Chloride, Crystal  4001
    Ammonium Sulfate  4027 Potassium Phosphate, Monobasic, Crystal  4008
    Boric Acid, Granular  4035 Potassium Phosphate, Dibasic, Powder  4012
    CAPS  4118 Sodium Acetate, Trihydrate, Crystal  4009
    Cesium Chloride  4042 Sodium Chloride, Crystal  4058
    CHAPS  4145 Sodium Citrate, Dihydrate, Granular  4093
    CHES  4146 Sodium Dodecyl Sulfate (SDS)  4095
    CHES, Sodium Salt  4147 Sodium Phosphate, Monobasic, Monohydrate, Crystal  4011
    1,4-Dithiothreitol (DTT, Cleland's Reagent)  F780 Sodium Phosphate, Dibasic, Anhydrous, Powder  4062
    EDTA, Disodium Salt, Dihydrate, Crystal  4040 Sucrose  4097
    Ethidium Bromide  4007 TEMED  4098
    Formamide  4028 TRIS (Base)  4109
    Glycerol, Anhydrous  4043 TRIS Hydrochloride  4103
    Glycine  4059 Urea  4111
    HEPES, Free Acid  4018 Water, Sterile  4221
    HEPES, Sodium Salt  4153    
    Lithium Chloride, Granular  4002    
    Magnesium Chloride, 6-Hydrate, Crystal  4003    

     

     ULTRAPURE Biological Buffer Selection Guide for Molecular Biology Applications 

    Description 

    pKa @25 °C 

    ∆pKa/∆T(°C) 

    pH Range 

    Product Number 

    CAPS, ULTRAPURE BIOREAGENT

    10.4

    -0.018

    9.7–11.1

    4118

    CHES, ULTRAPURE BIOREAGENT

    9.3

    -0.018

    8.6–10.0

    4146

    CHES, Sodium Salt, ULTRAPURE BIOREAGENT

    9.3

    -0.018

    8.6–10.0

    4147

    HEPES, Free Acid, ULTRAPURE BIOREAGENT

    7.5

    -0.014

    6.8–8.2

    4018

    HEPES, Sodium Salt, ULTRAPURE BIOREAGENT

    7.5

    -0.014

    6.8–8.2

    4153

    MES, Monohydrate, Free Acid, Crystalline, ULTRAPURE BIOREAGENT

    6.1

    -0.011

    5.5–6.7

    4014

    MOPS, ULTRAPURE BIOREAGENT

    7.2

    -0.011

    6.5–7.9

    4004

    MOPS, Sodium Salt, ULTRAPURE BIOREAGENT

    7.2

    -0.011

    6.5–7.9

    4163

    PIPES, ULTRAPURE BIOREAGENT

    6.8

    -0.0085

    6.1–7.5

    4265

    PIPES, Sodium Salt, ULTRAPURE BIOREAGENT

    6.8

    -0.0085

    6.1–7.5

    4266

    Tris, ULTRAPURE BIOREAGENT

    8.1

    -0.028

    7.0–9.0

    4109

    Tris, Biochemical Reagent

    8.1

    -0.028

    7.0–9.0

    4099

    Tris Hydrochloride, ULTRAPURE BIOREAGENT

    8.1

    -0.028

    7.0–9.0

    4103